Elisa Reader And Washer Principle

What an elisa reader does.

Elisa reader and washer principle.

An immune reaction i e. Elisa stands for enzyme linked immunosorbent assay. Along with the enzyme labelling of antigens or antibodies the technique involves following three principles in combination which make it one of the most specific and sensitive than other immunoassays to detect the biological molecule. The test is done in an elisa plate also known as a 96 well plate or microplate.

Are available as fixed as well as adjustable volume as well as single channel and multi channel. Readers need to have appropriate filter 650 nm and 450 nm. It can be manual system that washes one row or column at a time or semi automated systems that wash one strip or plate at a time or fully automated systems that can process multiple plates. In short it is an antibody test or a test for immune response to things attacking the body such as virus bacteria and allergens.

The illustration below shows a workflow for a typical sandwich elisa assay. Having the reactants of the elisa immobilized to the microplate surface enables easy separation of bound from non bound material during the assay. Elisa is a plate based assay technique. The elisa reader reads the plate.

The principle in elisa various antigen antibody combinations are used always including an enzyme labeled antigen or antibody and enzyme activity is measured colorimetrically. The enzyme activity is measured using a substrate that changes color when modified by the enzyme.